Journal: Journal of Biomedical Science

Article Title: Maternal causation of early-onset pre-eclampsia: excessive endometrial gland-derived apolipoprotein D induces placental ferroptosis and developmental abnormalities

doi: 10.1186/s12929-025-01199-7

Figure Lengend Snippet: Endometrial organoids from ePE and NT patients show impaired vascular remodeling and distinct molecular profiles. A Organoids display morphology and marker expression characteristic of endometrial glands. Immunofluorescence staining shows expressions of endometrial gland markers FOXA2 and PAX8. Scale bar = 50 µm. B Representative images demonstrate similari morphology size and quantity between NT and ePE organoids. Scale bar = 200 µm. C Comparable protein content in the organoid secretome between NT and ePE organoids. No significant differences were observed. Data are expressed as mean ± SD. N = 3. NS = not significant. D ePE organoid secretome inhibits endothelial cell angiogenesis. HUVECs (0.5 × 10 5 ) in Matrigel with organoid-conditioned medium and angiogenesis medium (1:1) for 16 h. Scale bar = 200 µm. Data are expressed as mean ± SD. N = 3. ** p < 0.01. E ePE organoid secretome impedes trophoblast invasion and uPA expression. EVTs (0.5 × 10 5 ) in Matrigel-coated Transwell inserts with organoid-conditioned media for invasion assay. uPA expression was measured by qPCR and shown as relative quantification (RQRQ = 2 −ΔΔCT ). Scale bar = 200 µm. Data are expressed as mean ± SD. N = 3. ** p < 0.01. F ePE organoid secretome reduces EVT integration into endothelial networks. HUVECs (0.5 × 10 5 , labeled green) seeded on Matrigel to form endothelial newtoek followed by EVTs (0.5 × 10 5 , labaeled red) with organoid-conditioned medium for 12 h.. Scale bar = 200 µm. Data are expressed as mean ± SD. N = 3. ** p < 0.01. G Venn diagram of differentially expressed genes (DEGs) between NT and ePE tissues and organoids. Decidua tissue, N = 3. Organoids, N = 4. H Principal Component Analysis (PCA) and hierarchical clustering analysis of DEGs in NT and ePE decidua tissues and organoids. I Venn diagram of differentiall expressed proteins (DEPs) in NT and ePE organoids (N = 5), DEG in NT and ePE organoids, and DEG in NT and ePE tissues. J Spatial transcriptome profiling of full thickness human endometrium gland showing glandular original of APOD colocalized with endometrial gland-specific marker PAEP

Article Snippet: Spatial transcriptome analysis of full-thickness endometrial sections was performed at the Beijing Genomics Institute-Shenzhen using our established protocols [ ].

Techniques: Marker, Expressing, Immunofluorescence, Staining, Invasion Assay, Quantitative Proteomics, Labeling